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KMID : 0616120070380020128
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Korean Journal of Pharmacognosy
2007 Volume.38 No. 2 p.128 ~ p.132
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(Variation of Saponin Content in the Decoctions of Platycodi Radix)
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Choi Jae-Seok
Yoo Dae-Seok
Choi Yeon-Hee
Yon Gyu-Hwan
Hong Kyung-Sik
Lee Byung-Hoi
Kim Hae-Jin
Kim Hyun-Gi
Kim Eun-Ju
No Sung-Hwan
Chung Young-Chul
Kim Young-Sup
Ryu Shi-Yong
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Abstract
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We investigated the contents of crude saponin in the decoctions of Platycodi Radix prepared by the different extraction processes. Each lyophilized decoctions prepared by the different protocol were suspended in water and partitioned with ethylacetate (EtOAc) and n-butanol (BuOH), which gave the EtOAc fraction, BuOH fraction and the remaining water fraction, respectively. The content of crude saponin, which was estimated as the amount of BuOH fraction, and the HPLC profiles of the BuOH fraction were analyzed, and compared each others. The crude saponin content in the decoctions were increased proportionally to the increment of the extraction time, amount of water, extraction temperature and the number of repetition for extraction. Consequently, the optimized conditions were deduced to be suitable and recommendable for the preparation of Platycodi Radix ; the water amount needed for the extraction be 15-20 volumes to crude material, temperature for extraction be optimal at 85{sim}100{circ}C, extraction time be more than 5 hours and the repetition of extraction was 2 times.
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KEYWORD
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Platycodi Radix, Campanulaceae, decoction, saponin content, HPLC
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